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Furthermore, during regeneration, the acidic and alkaline regenerants may corrode the vessel's rubber lining and the internal distributors (water caps)

Reactive Oxygen Species (ROS) levels were measured using DCFH-DA and MitoSOX Red, showing that metformin increased oxidative stress within the cells (69)

Zemba Cilic et al., 2021)

Author contributions CB-V, BB-M, and OV performed conceptualization, methodology, formal analysis, writing the original draft, and funding acquisition

However, careful attention to dosage, baseline Se status, and individual variability is essential to maximize benefits while avoiding toxicity